Interleukin mechanism in high fat diet

Considerable evidence has accumulated to suggest that the cytosolic ectopic accumulation of fatty acid metabolites, including diacylglycerol and ceramides, underlies the development of insulin resistance in skeletal muscle.

High-fat load: mechanism(s) of insulin resistance in skeletal muscle

Louis, MO was used to measure hepatic triglyceride secretion as described previously. Although a great deal has been learned about mechanisms underlying obesity-associated inflammation in peripheral tissues, much less is known about the pathogenesis of hypothalamic inflammation induced by HF feeding.

This article includes Supplementary Figures available at http: Determination of Liver Injury. Amounts of triglyceride, total cholesterol, and phospholipids were assayed enzymatically using kits obtained from Wako Pure Chemicals Co. Aspartate aminotransferase AST and alanine aminotransferase ALT activities in the serum were measured following a colorimetric procedure, using commercially available detection kits Jiancheng Institute of Biotechnology, Nanjing, China.

A Mice were weighed daily. Triglyceride levels in the supernatant of cultured hepatoytes were also measured C. These findings imply that obesity, or more likely the metabolic imbalance that causes obesity, leads to a progressive decline in mitochondrial function, eventually culminating in mitochondrial dissolution or mitoptosis.

In the arterial wall, IL-1 is secreted primarily by monocytes and macrophages but also by endothelial and smooth muscle cells [5][6].

We hypothesized that the pro-inflammatory cytokine interleukin-1 IL-1known to have an association with atherosclerosis, is the link between vascular responses and high fat feeding. Adult male mice were anesthetized by isoflurane inhalation, followed by a midline laparotomy.

Mice were then observed daily until postsurgical day 7 to determine survival. Gel Mobility Shift Assay. Supplementary material for this article can be found on the Hepatology website http: Materials and Methods Materials.

Semin Liver Dis ; However, no increase in ceramide content was detected in at least two other studies using lipid infusion to induce insulin resistance in rats or humans, 4647 and no differences in ceramide levels were found in the skeletal muscle from type 2 diabetic versus healthy individuals.

Dietary modification of macronutrient and salt intakes has also been studied with some large effects upon systolic blood pressure in particular [1] — [3].

IRS1 axis. Introduction Drugs to decrease plasma lipid levels and blood pressure are the basis of the medical treatment strategy for cardiovascular disease. After various time periods, mice were sacrificed, and serum levels of A triglyceride and B cholesterol were measured.

Blocking H2O2 emission prevents the shift in redox environment and preserves insulin sensitivity, providing evidence that the mitochondrial respiratory system is able to sense and respond to cellular metabolic imbalance.

Statistical significance was taken at the P less than. Mice were then sacrificed, A their livers were stained with HE, and B hepatic steatosis was scored. Interleukin IL has been reported to play a critical role in inflammatory progression of some liver diseases. As shown in Fig.

Abstract In peripheral tissues, the link between obesity and insulin resistance involves low-grade inflammation induced by macrophage activation and proinflammatory cytokine signaling.

Levels of IL-1 are increased in coronary arteries affected by atherosclerosis [10][11]and inhibition of IL-1 in animal models is associated with reduced amounts of atheroma [12] — [16] as well as neointima following angioplasty [17]. These methods are given in detail in the supplementary data.

Serum AST levels were measured. A gradual decrease in the sensitivity of skeletal muscle to insulin is considered a primary event in the disease process and, as such, likely holds the key to devising more effective prevention and treatment strategies.

Mice were then sacrificed, and hepatic lipid contents were measured. The system is tightly regulated by a natural antagonist, interleukin-1 receptor antagonist IL-1ra which also binds to IL-1R1 but does not produce a signal, and a non-signaling cell surface receptor, IL-1 receptor type-II, which acts as a decoy at the cell membrane or when shed as a soluble circulating decoy receptor [7][8].

We addressed this hypothesis by perturbing the inflammatory milieu of the hypothalamus in adult male Wistar rats using intracerebroventricular icv administration of interleukin-4 IL-4a Th2 cytokine that promotes alternative activation M2 of macrophages and microglia.

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IL-1 is an apical cytokine initiating inflammatory signals from bacterial products, chemical injury, complement activation and clotting factors.Fettreiche Ernährung erhöht das Melanomzellwachstum in der Knochenmarks-Nische durch Induktion von Osteopontin und Interleukin 6 High fat diet increases melanoma cell growth in the.

Interleukins, an appellation proposed inare immune modulators involved in critical biological functions, as well as in diseases,, and that have also been studied to develop therapies,,.

Interleukin-1 Regulates Multiple Atherogenic Mechanisms in Response to Fat Feeding

However, research on interleukins mainly focused on the immune aspects of these by: 2. Central administration of interleukin-4 exacerbates hypothalamic inflammation and weight gain during high-fat feeding Shinsuke Oh-I, * Joshua P.

Thaler, * Kayoko Ogimoto, Brent E. Wisse, Gregory J. Morton, and Michael W. SchwartzCited by:  · Interleukin Prevents Diet-Induced Insulin Resistance by Attenuating Macrophage and Cytokine Response in Skeletal Muscle Eun-Gyoung Hong 1, 2, Hwi Jin Ko 1, 3,Cited by: Anti-ILmAb administration in high-fat diet mice with IL axis blockade significantly improved liver function, attenuated hepatic lipid accumulation, suppressed Kuffer cells activation, and decreased proinflammatory cytokines levels, which were associated with inhibition of Cited by: 3.

As such, elevations in proinflammatory cytokines (tumor necrosis factor-α interleukin-6) and C-reactive protein have repeatedly been observed in the plasma, as well as within peripheral insulin target tissues (for example, liver, adipose tissue and skeletal muscle), of both animal (high-fat diet) and human (obesity and type 2 diabetes) models of insulin resistance (for review see de Luca and Olefsky 52 and Cited by: 9.

Interleukin mechanism in high fat diet
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